Conventional pyrolysis is a relatively simple way for biochar production; however, a single biomass feedstock approach is not promising for the development of multiple properties of biochar used in various applications. This study provides a detailed comparison in terms of pyrolysis kinetics between the cow manure and chicken manure, in addition to the co-pyrolysis by mixing the two livestock at different ratios. Eight different heating rates were tested, 5–40 °C/min with 5 °C/min step, on both livestock using the nitrogen as a gas agent with a flowrate of 50 ml/min. The initial results show that for the slow heating rates, 5 °C/min, the thermal degradation of the cow manure is different compared to that obtained from chicken manure. For the cow manure, the first peak, associated with hemicellulose decomposition, is higher than the second peak, associated with cellulose decomposition, which is the other way around for chicken manure. At 5 °C /min, the hemicellulose decomposition took place at 250 °C and 300 °C for the chicken manure and cow manure, respectively. The cellulose decomposition was started at 300 °C for chicken manure and 470 °C for cow manure. The lowest heat rate was selected for the co-pyrolysis case study to allow a quasi-equilibrium state to avoid measurement errors and heat transfer limitation in terms of thermal lag. Co-pyrolysis is studied with different blend ratios between the chicken and cow manure, where the 40% cow manure shows a positive result in terms of keeping an exothermic reaction over the co-pyrolysis process.