Cyclic mechanical strain has been demonstrated to enhance the development and function of engineered smooth muscle (SM) tissues, but appropriate scaffolds for engineering tissues under conditions of cyclic strain are currently lacking. These scaffolds must display elastic behavior, and be capable of inducing an appropriate smooth muscle cell (SMC) phenotype in response to mechanical signals. In this study, we have characterized several scaffold types commonly utilized in tissue engineering applications in order to select scaffolds that exhibit elastic properties under appropriate cyclic strain conditions. The ability of the scaffolds to promote an appropriate SMC phenotype in engineered SM tissues under cyclic strain conditions was subsequently analyzed. Poly(L-lactic acid)-bonded polyglycolide fiber-based scaffolds and type I collagen sponges exhibited partially elastic mechanical properties under cyclic strain conditions, although the synthetic polymer scaffolds demonstrated significant permanent deformation after extended times of cyclic strain application. SM tissues engineered with type I collagen sponges subjected to cyclic strain were found to contain more elastin than control tissues, and the SMCs in these tissues exhibited a contractile phenotype. In contrast, SMCs in control tissues exhibited a structure more consistent with the nondifferentiated, synthetic phenotype. These studies indicate the appropriate choice of a scaffold for engineering tissues in a mechanically dynamic environment is dependent on the time frame of the mechanical stimulation, and elastic scaffolds allow for mechanically directed control of cell phenotype in engineered tissues. [S0148-0731(00)00103-5]
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June 2000
Technical Papers
Scaffolds for Engineering Smooth Muscle Under Cyclic Mechanical Strain Conditions
Byung-Soo Kim, Graduate Student,,
Byung-Soo Kim, Graduate Student,
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109
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David J. Mooney, Associate Professor,
David J. Mooney, Associate Professor,
Departments of Chemical Engineering and Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109-2136
Search for other works by this author on:
Byung-Soo Kim, Graduate Student,
Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109
David J. Mooney, Associate Professor,
Departments of Chemical Engineering and Biologic and Materials Sciences, University of Michigan, Ann Arbor, MI 48109-2136
Contributed by the Bioengineering Division for publication in the JOURNAL OF BIOMECHANICAL ENGINEERING. Manuscript received by the Bioengineering Division October 21, 1999; revised manuscript received February 6, 2000. Associate Technical Editor: R. Vanderby, Jr.
J Biomech Eng. Jun 2000, 122(3): 210-215 (6 pages)
Published Online: February 6, 2000
Article history
Received:
October 21, 1999
Revised:
February 6, 2000
Citation
Kim, B., and Mooney, D. J. (February 6, 2000). "Scaffolds for Engineering Smooth Muscle Under Cyclic Mechanical Strain Conditions ." ASME. J Biomech Eng. June 2000; 122(3): 210–215. https://doi.org/10.1115/1.429651
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